On June 6, research teams such as Tomoyoshi Taka, a lecturer at the Graduate School of Medicine, Gunma University, announced that they have developed a method for acquiring both 23D microscopic images and 1D morphological images from a single biological specimen.
Observation with an optical microscope has been a common research method since the invention of the microscope in the 17th century.It is performed to analyze the morphology of cells and tissues, and to analyze the distribution of proteins and the like.At that time, the biological specimen is made into a very thin section and attached to a slide glass.However, the information obtained from the section is planar, and there is a drawback that the three-dimensional shape of the specimen and 3D information are lost.
This time, the research group has developed a device consisting of a cryostat, a single-lens reflex camera, and electronic work parts, which are devices that slice frozen specimens with a dedicated razor blade.In this device, the specimen moves up and down and the blade slices.The sliced sections are attached to a slide glass and observed under a microscope.At the same time, the cut surface of the specimen is photographed with a single-lens reflex camera.Take about 500 to 1000 shots with one specimen.This series of photos is reconstructed into a 3D image on your computer.
With this device, both 2D microscope images and 3D morphological images can be acquired, the information obtained from the sections is planar, and the problem of loss of the three-dimensional shape of the specimen and 3D information is solved.
In the experiment, specimens from plants, mice, stag beetles, and human brains were analyzed.It has the potential to spread in a wide range of fields in biological research, including medicine.
Paper information:[Scientific Reports] A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging
