By observing the amount of DNA of fish released into seawater, the technology to grasp how much fish live in the surrounding area is centered on Tetsushi Yamamoto, a researcher at the Graduate School of Human Development and Environmental Studies, Kobe University. It was developed for the first time in the world by a joint research group of 6 research institutes.This research result obtained by Kobe University, Hokkaido University, Institute of Statistical Mathematics, Kyoto University, Ryukoku University, and Chiba Prefectural Central Museum was published in the US online scientific journal "PLOS ONE" in March 2016.
On the other hand, a method for determining whether or not a fish to be examined inhabits from the DNA released into seawater together with the mucus secreted by the body has already been reported. In June 2014, a research group led by Dr. Yamamoto examined whether it is possible to clarify the location of fish and the size of its school by combining measurement of the amount of environmental DNA and a DNA fragment proliferation method called "real-time PCR method". ..
One liter of seawater was collected from the surface and bottom of the sea at 47 locations in Maizuru Bay in the northern part of Kyoto Prefecture, and the amount of environmental DNA of horse mackerel contained in it was analyzed. It was found that it was consistent with the distribution judgment and accurately captured the amount of horse mackerel.At the same time, it was proved that this technique is effective even in a wide sea area where the environmental DNA of fish was thought to spread.The survey in Maizuru Bay was conducted over a wide area of about 1 square kilometers, but the work that would take several days for capture and visual survey was completed in 11 hours.
If you know the DNA information of the fish you want to examine with this technology, you will be able to easily determine the presence or absence and amount of habitat in a short time.Furthermore, by applying this new technology, it is expected that the efficiency of investigating the distribution of fish stocks over a long period of time, such as year and season, will be dramatically improved.