A group of five high school students (at the time of research) from Shizuoka Prefectural Numazu Higashi High School, Shinichi Watanabe (currently Shizuoka Prefectural Ito Commercial High School), and Associate Professor Hideo Dora of the Shizuoka University Green Science and Technology Research Institute is in Suruga Bay. The intestinal flora of deep-sea fish collected from a depth of about 5 m was analyzed.
The intestinal flora, also called the intestinal flora, refers to a wide variety of bacterial groups that inhabit the intestines.Gut microbiota exist in a certain balance, and it is thought that adjusting this balance leads to maintenance of health and prevention of lifestyle-related diseases.Therefore, it is said that research on the intestinal flora of cultured fish is being conducted for the purpose of developing safe and secure aquaculture technology for fish, but so far the intestinal flora of deep-sea fish has been mostly analyzed worldwide. I didn't.
Based on the idea of high school students focusing on this point, in this joint research, DNA was extracted from the contents of the intestines of the deep-sea fish Aomeeso, Yumekasago, and Nigis collected from Suruga Bay, which boasts the deepest depth in Japan, and the intestines of deep-sea fish. The internal flora was analyzed for the first time with a next-generation sequencer.As a result, the intestinal flora of Aomeeso and Yumekasago was similar to the intestinal flora of fish reported so far, but in the intestinal flora of Nigis, the types of intestinal bacteria are significantly different. I understood.It was also found that in all three deep-sea fish, the abundant four genera occupy 3% of the intestinal flora, and the diversity of intestinal bacteria tends to be low.Further analysis may lead to an understanding of the relationship between the ecology of deep-sea fish and the intestinal flora.
The results of this research were published in the international academic journal "Microbiology Resource Announcements" published by the American Society for Microbiology, with Toshihide Iwatsuki, a high school student at the time, writing the paper as the lead author.In the future, it is expected to make a great contribution to understanding the characteristics of the intestinal bacterial flora of deep-sea fish and searching for novel marine microbial resources and genetic resources.
Paper information:[Microbiology Resource Announcements] 16S rRNA Gene Amplicon Sequencing of Gut Microbiota in Three Species of Deep-Sea Fish in Suruga Bay, Japan
