The placenta is an important organ that supplies nutrients and oxygen to the foetation.Placental stem cells (TS cells) are special placental-derived cells that have self-renewal ability and ability to differentiate into placental cells, and are expected to be useful tools for studying the development and function of human placenta. ..However, although a method for culturing mouse TS cells has already been established, it has been difficult to establish human TS cells.
The group of Assistant Professor Hiroaki Okae and Professor Takahiro Arima of Tohoku University will establish human TS cells from trophoblast stem cells, which are the main constituent cells of the human placenta, in collaboration with the group of Professor Hiroyuki Sasaki and Professor Mikita Suyama of Kyushu University. Especially successful for the first time in the world.
Cellular trophoblasts present in the human placenta have high proliferative and pluripotent potential.In this study, in order to understand how the proliferation of cellular trophoblasts is regulated in vivo, we isolated trophoblasts from human placenta with high purity and the genes that function in trophoblasts. Is said to have been comprehensively analyzed.As a result, factors that may control the proliferation of cellular trophoblasts were found, and when the culture conditions were examined based on this information, we succeeded in establishing human TS cells from cellular trophoblasts. bottom.
Human TS cells can be cultured for more than 5 months, and even after long-term culture, syncytial trophoblasts that work on hormone secretion and nutrient / gas exchange, and extrachorionic trophoblasts that reconstruct maternal blood vessels in the womb. Maintains the ability to differentiate into cells such as.The results of this research may be useful for elucidating the pathophysiology of diseases caused by the development and differentiation of human placenta and placental abnormalities, and for developing therapeutic methods, and are expected to contribute to reproductive medicine and regenerative medicine in the future. ing.
Paper information:[Cell Stem Cell] Derivation of Human Trophoblast Stem Cells
