According to Kinki University, fecal occult blood is a small amount of blood contained in stool and is not visible to the naked eye.It is seen when bleeding from the digestive tract due to gastric ulcer or colorectal cancer, but conventional reagents have problems such as expensive and unknown occult blood volume.

　The research group focused on luminol, which emits blue light when there is blood and is used in criminal investigations, and proceeded with the detection of fecal occult blood and the measurement of luminescence intensity using mice suffering from small intestinal ulcer.As a result, luminescence was confirmed by adding the luminol reagent to the stool collected from the mouse, and the blood volume could be measured by suspending a part of the stool with water and applying the supernatant and the luminol reagent to the measuring device. rice field.

　The fecal occult blood reagents currently in use have many challenges, and many researchers involved in animal experiments tend to rely on visual inspection.For this reason, mild fecal occult blood was often overlooked, but the detection method using the luminol reagent is inexpensive and can be detected immediately, enabling quantitative measurement.

　The research group believes that it will be the standard method for studying gastrointestinal ulcers and cancer in animal experiments.However, since luminol is affected by food-derived ingredients such as wasabi, it is necessary to solve this problem for human application.

Paper information:[Bio Techniques] Forensic luminol reaction for detecting fecal occult blood in experimental mice