This research group has so far revealed that not only bone mass and bone density but also bone matrix orientation strongly controls bone mechanical function.In particular, in the case of regenerated bone, we hypothesized that forming bone with a well-oriented bone matrix similar to that of healthy bone would be effective in preventing postoperative infections. We investigated the effect of the formation of phthalate on bacterial infection.

　When a unidirectional fine groove structure is created using metal 3D printing technology and osteoblasts are cultured on the groove substrate, the bone matrix constructed by the unidirectionally aligned osteoblasts also resembles biological bone. We have found that it is possible to achieve a regular orientation.By co-cultivating this with E. coli, we investigated how cell function affects the adhesion, survival, and proliferation of E. coli.

　As a result, they discovered that oriented osteoblasts significantly suppressed the adhesion of E. coli at an early stage.In addition, in the long term, we found that large amounts of β-defensin 2 and 3, a type of antibacterial protein, are produced, and by their ability to lyse E. coli, they exhibit high infection resistance and significantly reduce the number of E. coli. I understand.

　This research has demonstrated for the first time in the world that the healthy bone matrix orientation of bones not only increases bone mechanical strength, but also exhibits strong infection resistance from early to long-term bone formation.In other words, a bone device that induces bone matrix orientation is thought to be effective in promoting early and healthy bone healing and in suppressing postoperative infection.This result is expected to lead to the design of medical devices that enable early recovery from bone diseases.

Paper information:【Biomaterials Advances】Host bone microstructure for enhanced resistance to bacterial infections