On March 3, the research group of Professor Takuo Kubogi, Assistant Professor Masamitsu Oshima, Assistant Professor Mitsuaki Ohno, and Takashi Tsuji, Team Leader of RIKEN Center for Developmental Biology, Graduate School of Biomedical Sciences, Okayama University Announced that it demonstrated complete tooth regeneration in Beagle dogs using regenerating cell manipulation techniques.
In today's aging society, there is an increasing need to restore oral functions such as mastication and swallowing.When a tooth is lost, alternative treatments such as dentures, dentures, and implants are performed, but since it is inferior in terms of physiological function to natural teeth, regenerative treatment of the tooth itself is expected.
The research group has been developing techniques for regeneration of tooth germs and periodontal tissues in mice, but the application of these techniques to humans required success with large animals.
This time, the research group has established a technique for removing the permanent tooth germ of the first molar corresponding to the cap-shaped stage from the jawbone of a 55-day-old Beagle dog embryo, and conforms to the tooth germ reconstruction technique in a mouse model. To prepare a regenerated tooth germ.When a regenerated tooth germ derived from this canine tooth germ was transplanted under the renal capsule of an immunosuppressed mouse, it was revealed that regenerated teeth having a tissue structure equivalent to that of a natural tooth were generated at 8 weeks after transplantation.
Regenerated teeth are structurally and functionally equivalent to natural teeth, demonstrating practical tooth regeneration treatment in large animal models.
In the future, in order to apply this technology to humans and put it to practical use, it will be the biggest challenge to acquire cell seeds that enable tooth germ regeneration.Furthermore, in order to adapt to adults and the elderly, it is necessary to search for stem cells that can induce tooth germs.The research group will tackle these issues and aim for clinical practical application.
Published paper information:[Scientific Reports] Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model.
