Research groups at Fujita Medical University and Kyoto Prefectural University of Medicine have succeeded in developing a technique for producing skeletal muscle stem cells from fibroblasts and human iPS cells.

 Skeletal muscle stem cells have a mechanism in which when muscle regeneration is required, skeletal muscle is newly created and at the same time stem cells are produced again to prepare for the next regeneration.By applying this, for muscular dystrophy, which is one of the intractable muscle diseases, it is expected to develop a treatment that promotes regeneration by transplanting and replenishing stem cells, but usually in a state where it is not necessary to repair the muscle. There was the issue of how to collect non-proliferating skeletal muscular stem cells.

 So far, this research group has clarified that a transcription factor called Pax3 is strongly expressed when skeletal muscle stem cells proliferate.Therefore, in this study, we investigated transcription factors that are strongly expressed in addition to Pax3 among skeletal muscle stem cells that express mouse Pax3, and as a result, four types of PAX3, MYOD, HEYL, and KLF4 were Pax-positive from mouse fibroblasts. It was found to be an essential gene for inducing skeletal muscle stem cells.

 As a result, we succeeded in inducing PAX-positive skeletal muscle stem cells from mouse, human fibroblasts, and human iPS cells in about one month.When these induced human skeletal muscle stem cells were transplanted into muscular dystrophy model mice, it was confirmed that the abnormal expression of dystrophin, which causes muscular dystrophy, was dramatically recovered and greatly contributed to muscle regeneration.

 This result, which revealed that skeletal muscle stem cells that are the source of skeletal muscle can be produced using four types of genes, may lead to regenerative medicine by supplementing skeletal muscle stem cells in the future.The research group is aiming to develop a technology for proliferating and culturing a large amount of inducible human skeletal muscle stem cells.

Paper information:[Stem Cell Reports] Core transcription factors promote induction of PAX3-positive skeletal muscle stem cells

Kyoto Prefectural University of Medicine

Acquire rich humanity, professionalism, advanced and specialized knowledge and skills

Kyoto Prefectural University of Medicine is a public medical college that has a history of 1872 years since it was established in 140 (Meiji XNUMX) and boasts an old tradition. Based on the philosophy of "bringing the world's top-level medicine to the region," we are continuously training excellent medical professionals and medical scientists based on the synergies of intelligence, original creativity, and human power.Innovative base […]

Fujita Medical University

Politeness and kindness as a medical person, a medical person who will lead the next generation with advanced knowledge and technology

A comprehensive medical university with an educational, research, and medical environment on one campus.Acquire specialized knowledge and skills while touching cutting-edge medicine and medical care in a wide range of fields such as robotic surgery, robot rehabilitation, and community-based comprehensive care core center, as well as emergency medical care at four educational hospitals such as Fujita Medical University Hospital. What to do […]

University Journal Online Editorial Department

This is the online editorial department of the university journal.
Articles are written by editorial staff who have a high level of knowledge and interest in universities and education.