In the tissue diagnosis of cancer, the amount of cancer-related protein is calculated by an immunohistochemical method using an antigen-antibody reaction.There are limits to some of the immunohistochemical methods currently used, and the development of new test methods to improve the sensitivity, accuracy, and quantification of drug effects and prognosis diagnosis has become a major issue. ..

　To solve this problem, the research group developed a new method (one-particle imaging method of fluorescent nanoparticles) in 2015, measured the number of fluorescent nanoparticles bound to the target protein, and quantified the number of target proteins. However, the detection sensitivity was not sufficient.In addition, it was difficult to use this method at the same time as HE staining (staining method used for cell observation), which is indispensable for cancer tissue diagnosis.

　Therefore, we have developed a new fluorescent dyeing method that combines Tohoku University's one-particle imaging technology for fluorescent nanoparticles and Konica Minolta's material synthesis technology.As a result of applying this diagnostic method to the detection of cancer-related proteins in cancer tissues, we succeeded in accurately measuring the amount of the target protein with a detection sensitivity 1 times higher than that of the conventional method.Furthermore, as a result of actually applying this method to the cancer tissue diagnosis of breast cancer patients, we succeeded in accurately diagnosing and predicting the effect of drug therapy before treatment.

　With this new method, the effect of cancer molecular-targeted drugs can be quantitatively predicted, and it can be an index for selecting an anticancer drug suitable for patients. Therefore, future precision medicine (precision medicine) Is expected to contribute to.

Paper information:[Scientific Reports] Quantitative diagnostic imaging of cancer tissues by using phosphor-integrated dots with ultra-high brightness