A group of Keio University, Yasuda Women's University, Nagaoka University of Technology, Tokyo Institute of Technology and others has developed a technology to peel and generate cell sheets from general culture dishes and flasks simply by irradiating them with ultrasonic waves.
In normal cell ablation, enzymes are used to break down proteins that adhere cells to each other or to the culture surface, resulting in a single cell state.The separated cells are difficult to handle, and in regenerative medicine, low engraftment when transplanted into the body has been a problem.
On the other hand, the cell sheet, which is one of the basic technologies of regenerative medicine, is obtained by culturing cells using a special temperature-responsive culture dish, and when exposed to a low temperature environment, the cells are exfoliated from the culture surface in the form of a sheet.Cell sheets have contributed to the dramatic development of regenerative medicine because the efficiency of transplantation into the body is improved by the group of cultured cells connected in a sheet shape.However, there are also weaknesses such as "decrease in metabolism due to exposure of cells to a low temperature environment" and "an expensive and special culture dish is required as a consumable item".
Therefore, in this study, we aimed to generate cell sheets from general culture dishes under normal culture temperature (37 ° C) environment without the need for temperature reduction and without using chemicals, etc., and acoustic radiation pressure by ultrasonic waves. I came up with a method to physically detach cells using.
Based on this concept, when a device that propagates ultrasonic waves from below a normal culture dish to cells was manufactured and used, it was possible to generate a cell sheet derived from mouse skeletal muscle from a normal culture dish.It was found that this sheet has a higher metabolism than the sheet produced by the conventional method because it can be produced in a temperature environment suitable for culturing.Furthermore, by the same method, the peeling of the cell sheet from the flask was realized, which was unprecedented.
This technology can produce highly active cell sheets at low cost by maintaining the culture temperature and using a general culture vessel, and is expected to greatly contribute to the further development of regenerative medicine.
Paper information:[Scientific Reports] Detachment of cell sheets from clinically ubiquitous cell culture vessels by ultrasonic vibration
