A research group consisting of Professor Kei Miyamoto of the Faculty of Biophysical Engineering, Kinki University, Associate Professor Masayasu Yamada of the Graduate School of Agriculture, Kyoto University, and Professor John Gurdon of the Gardon Research Institute, University of Cambridge, has created a new method for dramatically improving the incidence of cloned animals. I found.By using this method, it becomes possible to easily and stably produce cloned mice from living cells.
Somatic cell cloning technology has been difficult to put into practical use because the incidence (clone efficiency) is less than 1%.In the same group, adult cells are stably reprogrammed by simply adding three compounds (tricostatin A, vitamin C, and deionized bovine serum albumin) to the culture medium for a specific period of time, in a specific combination, and in order. (Returning to the state of fertilized eggs), it was found that the incidence of clones increased by about 3%.Depending on the number of experiments, up to 15% of outbreaks were observed.
This stable initialization can be achieved only by changing the culture conditions.In other words, it is a simple method that can be applied to all animals, and it can be said that it has become possible to produce somatic cell cloned animals with extremely high efficiency.
In this research result, we were able to improve the efficiency of somatic cell cloning technology to a practical level, and this technology will be utilized for the conservation of endangered animals and the efficient production of medical model animals using genetically modified cells. It is expected to be done.
Paper information: [Biology Open] Reprogramming towards totipotency is greatly facilitated by synergistic effects of small molecules
