A joint research group of Ehime University, Aichi Gakuin University, and Yamaguchi University has developed a method of culturing fibroblasts by adding high-concentration trehalose to the culture medium, which has never been reported before, to create a senescence-like state that promotes wound healing. (Senescence-like State; SLS)” was discovered for the first time in the world.Using this discovery, we also showed that a dermal sheet containing high-concentration trehalose is an innovative product for regenerative medicine that can be expected to have a significantly higher wound healing effect.
Currently, there is no effective treatment for rapid regeneration of the damaged dermis in deep skin ulcers caused by peripheral blood flow disorders such as diabetes and varicose veins.On the other hand, senescence of cells refers to a state in which the cell cycle is stably arrested in response to DNA damage, etc., and is attracting attention as possibly playing an important role in wound healing.
In this study, the research group discovered for the first time in the world that an innovative phenomenon called SLS, which is very similar to senescence and favors wound healing, can be induced in fibroblasts by high-concentration trehalose treatment.When a dermal sheet prepared using human-derived fibroblasts and collagen was added with a high concentration of trehalose to temporarily induce SLS, it was confirmed that epidermal proliferation was significantly promoted on the dermal sheet. It is said thatAs a result of gene expression analysis, fibroblasts treated with a high concentration of trehalose showed significantly enhanced expression of growth factors that promote wound healing.Furthermore, animal experiments using mice also confirmed that dermal sheets containing high concentrations of trehalose promote wound healing of skin ulcers in mice.
From the above, it is suggested that the dermal sheet, which utilizes the SLS-inducing action of fibroblasts by high-concentration trehalose, may be useful for the treatment of deep-seated intractable ulcers, which have been difficult to treat until now. is expected to be put into practical use.
Paper information:[Communications Biology] Highly concentrated trehalose induces prohealing senescence-like state infibroblasts via CDKN1A/p21
